In the course of a public appearance to Lichfield Cathedral on the start of the week, the King was shouted at by a protester who asked him about Prince Andrew's links with disgraced financier Jeffrey Epstein.
The protester demanded to know how long the King had been aware about his sibling's association with Jeffrey Epstein, and also questioned whether he had instructed the authorities to "conceal" for Andrew.
Despite the heckling, there were crowds of well-wishers and cheers for the King, who was visiting Staffordshire for an event.
Prince Andrew's connections with Jeffrey Epstein have been under renewed focus in the past few weeks. The royal, who gave up his titles earlier this month, has repeatedly denied any misconduct.
"For how long have you known about Andrew and Jeffrey Epstein? Have you asked the police to cover up for Andrew?"
The monarch did not respond but carried on meeting supporters, some of whom chanted "God Save The King". One supporter can be overheard asking him for a embrace.
Another woman outside the venue was also observed telling the protester to "go away" and to "be quiet".
The scandal over Prince Andrew's connections to Jeffrey Epstein has been reignited by sexual abuse claims detailed in Virginia Giuffre's recently released book.
In the publication, Ms Giuffre says she had sex with the royal on three separate occasions, including once with Epstein and "eight other young girls"
Prince Andrew, who reached a financial settlement with Ms Giuffre in recent years, has consistently denied any wrongdoing. In a previous interview, he told a news program he did not remember meeting her "at all", and that they "did not have any sort of sexual contact."
But in recent times, fresh questions have been raised about how he is able to fund his lifestyle despite not being a working royal.
The revelation that he has only ever paid a nominal fee on his Royal Lodge has intensified the scrutiny over his housing situation - even though he made significant contributions initially, including for renovations.
The political party said he should be called in before MPs to give testimony about his lease of his lodge.
But last Thursday, the authorities refused to give MPs time to debate the prince's status or his residence, despite growing pressure for scrutiny.
The royal household has not commented on whether Prince Andrew might relocate of his home - or where he might go.
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